- Alois Trancy (Jim Phantomhive): Homosexual
- Cameron Gully: Homosexual
- Caesar Merritt: Homosexual
- Cassie Sandsmark: Homosexual
- Caroline Lexington: Bisexual
- Ciel Phantomhive: Bisexual
- Charlotte Garou: Homosexual
- Daniel Westley: ???
- Dominique Bretodeau: Bisexual
- Elvis: Bisexual
- Falchion A. Grey: Bisexual
- Farroel Faelad: Homosexual
- Geraldine Midford: ???
- Grell Sutcliffe: Transgender woman
- Helen Steele: Bisexual
- (Sir) Integra Fairbrook Wingates Hellsing: Asexual
- Kristopherson Miles: Homosexual
- Kyung-Soon Park: Homosexual
- Logan Kendrick: Bisexual
- Philip Faelad: Homosexual
- Roger Whinery: Transgender man
- Samantha Westley: Homosexual
- Sebastian Michaelis: Asexual
- Stephane Lexington: Bisexual
- Tegwen Sibyl: Homosexual
- Theodore "Ted" Ravenscroft: Bisexual
- Voros: Agender
- "Wink": Bisexual